By Thomas M. Bell (Auth.)
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Additional resources for An Introduction to General Virology
Gey, G. O. (1933) Amer. J. Cancer, 17, 752. 27. Salk, J. , Youngner, J. , Ward, E. N. (1954) Amer. J. Hyg. 60, 214. 28. Porterfield, J. S. (1960) Bull. Wld. Hlth. Org. 22, 373. 29. Cooper, P. D. (1961) Advances in Virus Research, 8, 319. CHAPTER SIX SEROLOGY THERE are five main types of serological reaction associated with viral investigations. Three of these, neutralisation, haemagglutinationinhibition (HI) and complement fixation (C-F) are used principally for diagnosis and epidemiology. The other two, agar-gel diffusion and fluorescent antibody staining are primarily research tools, only now well enough understood by all virologists for tentative diagnostic application.
11. , Burke, D. C. (1959) Brit. Med. Bull. 15, 185. 12. Burke, D. C. (1961) Biochem. J. 78, 556. 13. , Cantell, K. (1962) Virology, 18, 145. 14. Wagner, R. R. (1961) Virology, 13, 323. 15. Ho, M. (1961) Proc. Soc. Exp. Biol. Med. 107, 639. 16. , Schonne, E. (1962) Virology, 16, 63. 17. , Enders, J. F. (1959) Virology, 9, 446. 18. Chany, C. (1960) Compt. Rend. Acad. Sei. 250, 3903. 19. Cantell, K. ges. Virusforch. 10, 510. 20. , Porterfield, J. , Baron, S. (1961) Virology, 14, 450. 21. Warburg, O.
Then the trypsin solution is passed through three layers of sterile cheese cloth to remove tissue particles and connective fibres. Fresh trypsin is added to the minced fragments in the stirrer and the process is repeated 8-10 times, pooling all the supernatant fluids. The resulting suspension of small clumps and single cells is then centrifuged at 1000 g for 5 minutes and the sediment suspended in approximately 20 volumes of medium (2 % horse serum in 199). out at 600 g, resuspended and centrifuged twice more before final suspension as a 1:50 dilution based on the packed cell volume.
An Introduction to General Virology by Thomas M. Bell (Auth.)